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髓核致炎大鼠背根神经节中NF-资B和ASICs上调机制的探讨
朱杭, 林士明, 王栋, 诸力, 何永江, 王坚, 潘浩
浙江中医药大学附属广兴医院骨科
摘要:
目的通过观察核因子-资B(NF-资B)和酸敏感离子通道(ASIC)信号通路阻断剂PDTC和阿米洛利对髓核致炎大鼠背根神经节(DRG)中NF-资B和ASIC3表达的影响,探索NF-资B和ASIC3在髓核组织诱导神经根性疼痛的可能机制。方法将80只大鼠采用随机数字表法分为5组,对照组、模型组、阿米洛利组、PDTC组和阿米洛利组+PDTC组,每组16只。对照组在暴露L5DRG后直接缝合。模型组取出大鼠尾部的髓核组织置于暴露的L5DRG上,缝合切口。阿米洛利组和PDTC组在模型组的基础上,分别于术后2周内每天腹腔内注射20滋g/kg阿米洛利和20滋g/kgPDTC。阿米洛利+PDTC组在模型组基础上,术后2周内每天腹腔内注射20滋g/kg阿米洛利和20滋g/kgPDTC。对照组和模型组不进行药物干预。造模成功后分别于术后2、4、6、8、10、12d检测大鼠后足机械刺激缩足阈值(PWT)。采用RT-PCR法和Westernblot法测定ASIC3、NF-资Bp65、TNF-α和IL-6mRNA和蛋白表达水平。术后1、3、7、14d采用ELISA法检测外周血及DRG上清液中的TNF-α和IL-6水平,并检测一氧化氮(NO)水平。结果与对照组比较,术后12d时模型组中大鼠后足的PWT显著下降(P<0.01),术后14d模型组DRG中NF-资Bp65和ASIC3的mRNA和蛋白表达显著上调(均P<0.05),外周血和DRG中NO、TNF-α、IL-6水平均显著上调(均P<0.01)。与模型组比较,阿米洛利组、PDTC组和阿米洛利+PDTC组大鼠后足PWT在8d内下降,之后逐渐增加,术后10、12d大鼠后足PWT明显高于模型组(均P<0.01),术后14dDRG中NF-资Bp65和ASIC3的mRNA和蛋白表达明显下降(均P<0.05),外周血和DRG中NO、TNF-琢、IL-6水平均显著降低(均P<0.01)。结论NF-资B和ASIC3可相互影响,通过抑制NO及多种细胞炎性因子(TNF-琢和IL-6)的表达,减轻髓核组织对DRG的炎症损伤所致的痛觉过敏,减缓神经根性疼痛。
关键词:  宫内发育 新生儿 小于胎龄儿 适于胎龄儿 大于胎龄儿
DOI:10.12056/j.issn.1006-2785.2018.40.2.2017-953
分类号:
基金项目:
Factors related to birth weight for gestational age
Guangxing Hosptial Affiliated to Zhejiang Medicine University
Abstract:
Objective To analyze the factors related to birth weight for gestational age. Methods Total 693 pregnant mothers and their 716 newborns were enrolled in the study. The age, height, weight and pregnancy details of parents were collected by questionnaire. The birth information of the newborn was completed by obstetricians, including parity, gestational age, birth weight, number of fetus and gender. According to the gestational age and birth weight, the newborns were divided into small for gestational age (SGA) group(n=115), appropriate for gestational age(AGA) group(n=330) and large for gestational age(LGA) group (n=271), the factors related to birth weight for gestational age were analyzed. Results The differences of gender, gestational age, birth weight, premature/mature/postmature, number of fetus and parity; the age, height, weight and BMI of fathers; the age, height, pre-gestational weight, prenatal weight, weight gain during pregnancy, percentage of weight gain during pregnancy, pre-gestational BMI, prenatal BMI, BMI gain during pregnancy, percentage of BMI gain during pregnancy and the proportion of gestational diabetes mellitus (GDM) of mothers among three groups were significant (all P <0.05). Pearson correlation analysis showed that the birth weight for gestational age was positively correlated with the age, height, weight, BMI of fathers; and the age, height, pre-gestational weight, prenatal weight, weight gain during pregnancy, pre-gestational BMI, prenatal BMI, BMI gain during pregnancy, gravida and parity of mothers (all P<0.05); and negatively correlated with gender and number of fetus (both P <0.05). The multiple linear regression analysis showed that the birth weight for gestational age was negatively correlated with number of fetus and gender, and positively correlated with the height, BMI of fathers; the age, height, ratio of BMI gain during pregnancy, prenatal BMI, parity of mothers (all P<0.05). Conclusion Based on the results revealed in the study, for the health of newborns it is necessary to rationally control the nutrition of pregnant women, and avoid the maternal malnutrition or over weight gain during pregnancy.
Key words:  Intrauterine growth Newborn Small for gestational age Appropriate for gestational age Large for gestational age